• Upper Extremity Surgery Division / Research

• Results

Upper Extremity Surgery Division

Three senior staff and four PhD students are conducting basic and clinical research on shoulder, elbow, and hand surgery. The main topics are tendon biology, tendinosis pathology, and its regeneration. We are also interested in outcomes of massive rotator cuff reconstruction using autologous fascia graft and reversed shoulder arthroplasty.

[Clinical Research]

Evaluation of shoulder muscles and tendons using ultrasound
Ultrasonography is a real-time, noninvasive technique to assess muscle and tendon. We are analyzing those structures and biomechanical characteristics under pathological conditions.
Quantification of neuralgic pain
Pain is one of most difficult symptoms assessed subjectively. We are using a pain-quantitating device and analyze its association with other parameters.

[Basic Research]

Pathology of tendon/rotator cuff under aging, oxidative stress
Accumulation of oxidative stress during aging is one of the causes of tendinosis. We are analyzing these factors in in vivo and in vitro tendinosis models.
Rotator cuff regeneration by tissue engineering
Scaffold, growth factor, and stem cells are key factors for successful tendon tissue regeneration. We are focusing on absorbable biomaterial and CELL SHEET to treat massive rotator cuff tears.
Pathology of muscle atrophy
The muscle weakness after nerve or tendon injury negatively affects the clinical outcomes. Analysis of muscle progenitor cell and controlling it is one of the key factors to obtain good clinical results.

• Upper Extremity Surgery Division / Results

• Research

Original Research Article

(1) Yamaura K, Mifune Y, Inui A, Nishimoto H, Mukohara S, Yoshikawa T, Shinohara I, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Novel therapy using a fish scale collagen scaffold for rotator cuff healing in rat models. J Shoulder Elbow Surg. 2022;31(12):2629-2637.

(2) Tanaka S, Inui A, Mifune Y, Nishimoto H, Yoshikawa T, Shinohara I, Furukawa T, Kato T, Kusunose M, Kuroda R. Motion Analysis of the Extensor Carpi Ulnaris in Triangular Fibrocartilage Complex Injury Using Ultrasonography Images. Sensors (Basel). 2022 ;22(21):8216.

(3) Shinohara I, Inui A, Mifune Y, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Using deep learning for ultrasound images to diagnose carpal tunnel syndrome with high accuracy. Ultrasound Med Biol. 2022;48(10):2052-2059.

(4) Yamaura K, Inui A, Mifune Y, Mukohara S, Furukawa T, Kuroda R. Efficacy of Abductor Pollicis Longus Suspension Arthroplasty Combined With Mini TightRope for Osteoarthritis of Thumb Carpometacarpal Joint. Hand (N Y). 2022 Sep 16:15589447221120849. doi: 10.1177/15589447221120849.

(5) Harada R, Taniguchi-Ikeda M, Nagasaka M, Nishii T, Inui A, Yamamoto T, Morioka I, Kuroda R, Iijima K, Nozu K, Sakai Y, Toda T. Assessment of the upper limb muscles in patients with Fukuyama muscular dystrophy: Noninvasive assessment using visual ultrasound muscle analysis and shear wave elastography. Neuromuscul Disord. 2022 ;32(9):754-762.

(6) Shinohara I, Inui A, Mifune Y, Nishimoto H, Mukohara S, Yoshikawa T, Kuroda R. Ultrasound With Artificial Intelligence Models Predicted Palmer 1B Triangular Fibrocartilage Complex Injuries. Arthroscopy. 2022;38(8):2417-2424.

(7) Yoshikawa T, Mifune Y, Inui A, Nishimoto H, Yamaura K, Mukohara S, Shinohara I, Kuroda R. Quercetin treatment protects the Achilles tendons of rats from oxidative stress induced by hyperglycemia. BMC Musculoskelet Disord. 2022;23(1):563.

(8) Shinohara I, Mifune Y, Inui A, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Biochemical Markers of Aging (Advanced Glycation End Products) and Degeneration Are Increased in Type 3 Rotator Cuff Tendon Stumps With Increased Signal Intensity Changes on MRI. Am J Sports Med. 2022 (7):1960-1970.

(9) Yoshikawa T, Mifune Y, Inui A, Nishimoto H, Yamaura K, Mukohara S, Shinohara I, Kuroda R. Influence of Diabetes-Induced Glycation and Oxidative Stress on the Human Rotator Cuff. Antioxidants (Basel). 2022;11(4):743.

(10) Shinohara I, Mifune Y, Inui A, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Advanced glycation end products are associated with limited range of motion of the shoulder joint in patients with rotator cuff tears associated with diabetes mellitus. BMC Musculoskelet Disord. 2022;23(1):271.

(11) Yamaura K, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Mukohara S, Hoshino Y, Niikura T, Kuroda R. Antioxidant effect of nicotinamide mononucleotide in tendinopathy. BMC Musculoskelet Disord. 2022;23(1):249.

(12) Shinohara I, Inui A, Mifune Y, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Diagnosis of Cubital Tunnel Syndrome Using Deep Learning on Ultrasonographic Images. Diagnostics (Basel). 2022;12(3):632.

(13) Nagura I, Kanatani T, Harada Y, Takase F, Inui A, Mifune Y, Kuroda R. Preoperative Evaluation of Thenar Muscles in Carpal Tunnel Syndrome by Ultrasonography. Hand Clin. 2022 ;38(1):55-58.

(14) Yamaura K, Mifune Y, Inui A, Nishimoto H, Kataoka T, Kurosawa T, Mukohara S, Hoshino Y, Niikura T, Nagamune K, Kuroda R. Accuracy and reliability of tridimensional electromagnetic sensor system for elbow ROM measurement. J Orthop Surg Res. 2022;17(1):60.

(15) Shinohara I, Kataoka T, Mifune Y, Inui A, Sakata R, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Matsushita T, Kuroda R. Influence of adiponectin and inflammatory cytokines in fatty degenerative atrophic muscle. Sci Rep. 2022;12(1):1557.

(16) Shinohara I, Inui A, Mifune Y, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Motion Analysis of Triangular Fibrocartilage Complex by Using Ultrasonography Images: Preliminary Analysis. Sensors (Basel). 2022;22(1):345.

(17) Kataoka T, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Yamaura K, Mukohara S, Matsushita T, Niikura T, Tabata Y, Kuroda R. Combined therapy of platelet-rich plasma and basic fibroblast growth factor using gelatin-hydrogel sheet for rotator cuff healing in rat models. J Orthop Surg Res. 2021 Oct 16;16(1):605.

(18) Yamaura K, Mifune Y, Inui A, Nishimoto H, Kataoka T, Kurosawa T, Mukohara S, Niikura T, Kokubu T, Kuroda R. Sequential Changes in Posterior Shoulder Muscle Elasticity After Throwing as Assessed via Ultrasound Shear Wave Elastography. Orthop J Sports Med. 2021 Aug 11;9(8):23259671211021362.

(19) Mukohara S, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Yamaura K, Yoshikawa T, Shinohara I, Hoshino Y, Nagamune K, Kuroda R. A new quantitative evaluation system for distal radioulnar joint instability using a three-dimensional electromagnetic sensor. J Orthop Surg Res. 2021 Jul 14;16(1):452.

(20) Mukohara S, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Yamaura K, Yoshikawa T, Waki T, Kuroda T, Niikura T, Kuroda R. Effects of trochlear fragmentation on functional outcome in coronal shear fractures: a retrospective comparative study. JSES Int. 2021 Feb 20;5(3):571-577.

(21) Mukohara S, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Yamaura K, Yoshikawa T, Kuroda R. In vitro and in vivo tenocyte-protective effectiveness of dehydroepiandrosterone against high glucose-induced oxidative stress. BMC Musculoskelet Disord. 2021 Jun 5;22(1):519.

(22) Yamaura K, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Mukohara S, Yoshikawa T, Hoshino Y, Niikura T, Kokubu T, Kuroda R. Relationship between glenohumeral internal rotation deficit and shoulder conditions in professional baseball pitchers. J Shoulder Elbow Surg. 2021 Sep;30(9):2073-2081.

(23) Inui A, Mifune Y, Nishimoto H, Niikura T, Kuroda R. Reconstruction of Carpal Bone Loss of Septic Wrist Arthritis Using Induced Membrane Technique. J Hand Microsurg. 2020 12:S54-S57.

(24) Kokubu T, Mifune Y, Kanzaki N, Hoshino Y, Kakutani K, Inui A, Hashimoto S, Kuroda R. Muscle Strains in the Lower Extremity of Japanese Professional Baseball Players. Orthop J Sports Med. 2020;8(10):2325967120956569.

(25) Harada Y, Inui A, Mifune Y, Nishimoto H, Kokubu T, Hiroyuki F, Kuroda R. Treatment of Locking of the Metacarpophalangeal Joint of the Thumb. J Hand Microsurg. 2020;12(1):62-66.

(26) Ueda Y, Inui A, Mifune Y, Takase F, Kataoka T, Kurosawa T, Yamaura K, Kokubu T, Kuroda R. Molecular changes to tendons after collagenase-induced acute tendon injury in a senescence-accelerated mouse model.　BMC Musculoskelet Disord. 2019;20(1):120

(27) Mifune Y, Inui A, Muto T, Nishimoto H, Kataoka T, Kurosawa T, Yamaura K, Mukohara S, Niikura T, Kokubu T, Kuroda R. Influence of advanced glycation end products on rotator cuff. J Shoulder Elbow Surg. 2019;28(8):1490-1496

(28) Mifune Y, Inui A, Nishimoto H, Kataoka T, Kurosawa T, Yamaura K, Mukohara S, Niikura T, Kokubu T, Akisue T, Kuroda R. Assessment of posterior shoulder muscle stiffness related to posterior shoulder tightness in college baseball players using shear wave elastography. J Shoulder Elbow Surg. 2019; doi: 10.1016/j.jse.2019.05.028

(29) Inui A, Kuroda T, Kurosawa T, kokubu T, Mifune Y, Nishimoto H, Kuroda R. Case Series of Comminuted Olecranon Fracture Treated by Plate Fixation; Do We Have to Remove the Plate? Kobe J Med Sci, 64, 115-118, 2018

(30) Kataoka T, Kokubu T, Muto T, Mifune Y, Inui A, Sakata R, Nishimoto H, Harada Y, Takase F, Ueda Y, Kurosawa T, Yamaura K, Kuroda R. Rotator cuff tear healing process with graft augmentation of fascia lata in a rabbit model. J Orthop Surg Res. 2018 Aug 13;13(1):200. doi: 10.1186/s13018-018-0900-4.

(31) Ueda Y, Inui A, Mifune Y, Sakata R, Muto T, Harada Y, Takase F, Kataoka T, Kokubu T, Kuroda R. The effects of high glucose condition on rat tenocytes in vitro and rat Achilles tendon in vivo. Bone Joint Res. 2018 Jun 5;7(5):362-372.

(32) Takase F, Mifune Y, Inui A, Ueda Y, Kataoka T, Kokubu T, Kuroda R. Association of advanced glycation end products in Dupuytren disease. J Orthop Surg Res. 2018 Jun 7;13(1):143. doi: 10.1186/s13018-018-0848-4.

• Upper Extremity Surgery Division / Research

• Results

Upper Extremity Surgery Division

Three senior staff and four PhD students are conducting basic and clinical research on shoulder, elbow, and hand surgery. The main topics are tendon biology, tendinosis pathology, and its regeneration. We are also interested in outcomes of massive rotator cuff reconstruction using autologous fascia graft and reversed shoulder arthroplasty.

[Clinical Research]

Evaluation of shoulder muscles and tendons using ultrasound
Ultrasonography is a real-time, noninvasive technique to assess muscle and tendon. We are analyzing those structures and biomechanical characteristics under pathological conditions.
Quantification of neuralgic pain
Pain is one of most difficult symptoms assessed subjectively. We are using a pain-quantitating device and analyze its association with other parameters.

[Basic Research]

Pathology of tendon/rotator cuff under aging, oxidative stress
Accumulation of oxidative stress during aging is one of the causes of tendinosis. We are analyzing these factors in in vivo and in vitro tendinosis models.
Rotator cuff regeneration by tissue engineering
Scaffold, growth factor, and stem cells are key factors for successful tendon tissue regeneration. We are focusing on absorbable biomaterial and CELL SHEET to treat massive rotator cuff tears.
Pathology of muscle atrophy
The muscle weakness after nerve or tendon injury negatively affects the clinical outcomes. Analysis of muscle progenitor cell and controlling it is one of the key factors to obtain good clinical results.

• Upper Extremity Surgery Division / Results

• Research

Original Research Article

(1) Yamaura K, Mifune Y, Inui A, Nishimoto H, Mukohara S, Yoshikawa T, Shinohara I, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Novel therapy using a fish scale collagen scaffold for rotator cuff healing in rat models. J Shoulder Elbow Surg. 2022;31(12):2629-2637.

(2) Tanaka S, Inui A, Mifune Y, Nishimoto H, Yoshikawa T, Shinohara I, Furukawa T, Kato T, Kusunose M, Kuroda R. Motion Analysis of the Extensor Carpi Ulnaris in Triangular Fibrocartilage Complex Injury Using Ultrasonography Images. Sensors (Basel). 2022 ;22(21):8216.

(3) Shinohara I, Inui A, Mifune Y, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Using deep learning for ultrasound images to diagnose carpal tunnel syndrome with high accuracy. Ultrasound Med Biol. 2022;48(10):2052-2059.

(4) Yamaura K, Inui A, Mifune Y, Mukohara S, Furukawa T, Kuroda R. Efficacy of Abductor Pollicis Longus Suspension Arthroplasty Combined With Mini TightRope for Osteoarthritis of Thumb Carpometacarpal Joint. Hand (N Y). 2022 Sep 16:15589447221120849. doi: 10.1177/15589447221120849.

(5) Harada R, Taniguchi-Ikeda M, Nagasaka M, Nishii T, Inui A, Yamamoto T, Morioka I, Kuroda R, Iijima K, Nozu K, Sakai Y, Toda T. Assessment of the upper limb muscles in patients with Fukuyama muscular dystrophy: Noninvasive assessment using visual ultrasound muscle analysis and shear wave elastography. Neuromuscul Disord. 2022 ;32(9):754-762.

(6) Shinohara I, Inui A, Mifune Y, Nishimoto H, Mukohara S, Yoshikawa T, Kuroda R. Ultrasound With Artificial Intelligence Models Predicted Palmer 1B Triangular Fibrocartilage Complex Injuries. Arthroscopy. 2022;38(8):2417-2424.

(7) Yoshikawa T, Mifune Y, Inui A, Nishimoto H, Yamaura K, Mukohara S, Shinohara I, Kuroda R. Quercetin treatment protects the Achilles tendons of rats from oxidative stress induced by hyperglycemia. BMC Musculoskelet Disord. 2022;23(1):563.

(8) Shinohara I, Mifune Y, Inui A, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Biochemical Markers of Aging (Advanced Glycation End Products) and Degeneration Are Increased in Type 3 Rotator Cuff Tendon Stumps With Increased Signal Intensity Changes on MRI. Am J Sports Med. 2022 (7):1960-1970.

(9) Yoshikawa T, Mifune Y, Inui A, Nishimoto H, Yamaura K, Mukohara S, Shinohara I, Kuroda R. Influence of Diabetes-Induced Glycation and Oxidative Stress on the Human Rotator Cuff. Antioxidants (Basel). 2022;11(4):743.

(10) Shinohara I, Mifune Y, Inui A, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Advanced glycation end products are associated with limited range of motion of the shoulder joint in patients with rotator cuff tears associated with diabetes mellitus. BMC Musculoskelet Disord. 2022;23(1):271.

(11) Yamaura K, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Mukohara S, Hoshino Y, Niikura T, Kuroda R. Antioxidant effect of nicotinamide mononucleotide in tendinopathy. BMC Musculoskelet Disord. 2022;23(1):249.

(12) Shinohara I, Inui A, Mifune Y, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Diagnosis of Cubital Tunnel Syndrome Using Deep Learning on Ultrasonographic Images. Diagnostics (Basel). 2022;12(3):632.

(13) Nagura I, Kanatani T, Harada Y, Takase F, Inui A, Mifune Y, Kuroda R. Preoperative Evaluation of Thenar Muscles in Carpal Tunnel Syndrome by Ultrasonography. Hand Clin. 2022 ;38(1):55-58.

(14) Yamaura K, Mifune Y, Inui A, Nishimoto H, Kataoka T, Kurosawa T, Mukohara S, Hoshino Y, Niikura T, Nagamune K, Kuroda R. Accuracy and reliability of tridimensional electromagnetic sensor system for elbow ROM measurement. J Orthop Surg Res. 2022;17(1):60.

(15) Shinohara I, Kataoka T, Mifune Y, Inui A, Sakata R, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Matsushita T, Kuroda R. Influence of adiponectin and inflammatory cytokines in fatty degenerative atrophic muscle. Sci Rep. 2022;12(1):1557.

(16) Shinohara I, Inui A, Mifune Y, Nishimoto H, Yamaura K, Mukohara S, Yoshikawa T, Kato T, Furukawa T, Hoshino Y, Matsushita T, Kuroda R. Motion Analysis of Triangular Fibrocartilage Complex by Using Ultrasonography Images: Preliminary Analysis. Sensors (Basel). 2022;22(1):345.

(17) Kataoka T, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Yamaura K, Mukohara S, Matsushita T, Niikura T, Tabata Y, Kuroda R. Combined therapy of platelet-rich plasma and basic fibroblast growth factor using gelatin-hydrogel sheet for rotator cuff healing in rat models. J Orthop Surg Res. 2021 Oct 16;16(1):605.

(18) Yamaura K, Mifune Y, Inui A, Nishimoto H, Kataoka T, Kurosawa T, Mukohara S, Niikura T, Kokubu T, Kuroda R. Sequential Changes in Posterior Shoulder Muscle Elasticity After Throwing as Assessed via Ultrasound Shear Wave Elastography. Orthop J Sports Med. 2021 Aug 11;9(8):23259671211021362.

(19) Mukohara S, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Yamaura K, Yoshikawa T, Shinohara I, Hoshino Y, Nagamune K, Kuroda R. A new quantitative evaluation system for distal radioulnar joint instability using a three-dimensional electromagnetic sensor. J Orthop Surg Res. 2021 Jul 14;16(1):452.

(20) Mukohara S, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Yamaura K, Yoshikawa T, Waki T, Kuroda T, Niikura T, Kuroda R. Effects of trochlear fragmentation on functional outcome in coronal shear fractures: a retrospective comparative study. JSES Int. 2021 Feb 20;5(3):571-577.

(21) Mukohara S, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Yamaura K, Yoshikawa T, Kuroda R. In vitro and in vivo tenocyte-protective effectiveness of dehydroepiandrosterone against high glucose-induced oxidative stress. BMC Musculoskelet Disord. 2021 Jun 5;22(1):519.

(22) Yamaura K, Mifune Y, Inui A, Nishimoto H, Kurosawa T, Mukohara S, Yoshikawa T, Hoshino Y, Niikura T, Kokubu T, Kuroda R. Relationship between glenohumeral internal rotation deficit and shoulder conditions in professional baseball pitchers. J Shoulder Elbow Surg. 2021 Sep;30(9):2073-2081.

(23) Inui A, Mifune Y, Nishimoto H, Niikura T, Kuroda R. Reconstruction of Carpal Bone Loss of Septic Wrist Arthritis Using Induced Membrane Technique. J Hand Microsurg. 2020 12:S54-S57.

(24) Kokubu T, Mifune Y, Kanzaki N, Hoshino Y, Kakutani K, Inui A, Hashimoto S, Kuroda R. Muscle Strains in the Lower Extremity of Japanese Professional Baseball Players. Orthop J Sports Med. 2020;8(10):2325967120956569.

(25) Harada Y, Inui A, Mifune Y, Nishimoto H, Kokubu T, Hiroyuki F, Kuroda R. Treatment of Locking of the Metacarpophalangeal Joint of the Thumb. J Hand Microsurg. 2020;12(1):62-66.

(26) Ueda Y, Inui A, Mifune Y, Takase F, Kataoka T, Kurosawa T, Yamaura K, Kokubu T, Kuroda R. Molecular changes to tendons after collagenase-induced acute tendon injury in a senescence-accelerated mouse model.　BMC Musculoskelet Disord. 2019;20(1):120

(27) Mifune Y, Inui A, Muto T, Nishimoto H, Kataoka T, Kurosawa T, Yamaura K, Mukohara S, Niikura T, Kokubu T, Kuroda R. Influence of advanced glycation end products on rotator cuff. J Shoulder Elbow Surg. 2019;28(8):1490-1496

(28) Mifune Y, Inui A, Nishimoto H, Kataoka T, Kurosawa T, Yamaura K, Mukohara S, Niikura T, Kokubu T, Akisue T, Kuroda R. Assessment of posterior shoulder muscle stiffness related to posterior shoulder tightness in college baseball players using shear wave elastography. J Shoulder Elbow Surg. 2019; doi: 10.1016/j.jse.2019.05.028

(29) Inui A, Kuroda T, Kurosawa T, kokubu T, Mifune Y, Nishimoto H, Kuroda R. Case Series of Comminuted Olecranon Fracture Treated by Plate Fixation; Do We Have to Remove the Plate? Kobe J Med Sci, 64, 115-118, 2018

(30) Kataoka T, Kokubu T, Muto T, Mifune Y, Inui A, Sakata R, Nishimoto H, Harada Y, Takase F, Ueda Y, Kurosawa T, Yamaura K, Kuroda R. Rotator cuff tear healing process with graft augmentation of fascia lata in a rabbit model. J Orthop Surg Res. 2018 Aug 13;13(1):200. doi: 10.1186/s13018-018-0900-4.

(31) Ueda Y, Inui A, Mifune Y, Sakata R, Muto T, Harada Y, Takase F, Kataoka T, Kokubu T, Kuroda R. The effects of high glucose condition on rat tenocytes in vitro and rat Achilles tendon in vivo. Bone Joint Res. 2018 Jun 5;7(5):362-372.

(32) Takase F, Mifune Y, Inui A, Ueda Y, Kataoka T, Kokubu T, Kuroda R. Association of advanced glycation end products in Dupuytren disease. J Orthop Surg Res. 2018 Jun 7;13(1):143. doi: 10.1186/s13018-018-0848-4.