Kobe Journal of Medical Sciences, 2000

IMMUNOHISTOCHEMICAL STUDY OF P53, PCNA AND AFP IN HEPATOCELLULAR CARCINOMA, A COMPARISON BETWEEN INDONESIAN AND JAPANESE CASES. 

Wirasmi MARWOTO,1 Upik Anderiani MISKAD,2 Nuryati Chaerani SIREGAR,1 Rino A. GANI,3 Unggul BOEDIHUSODO,3 Siti NURDJANAH,1 SUWARSO,5 WATADIANTO,6 Poernomo BOEDI,7 H.A . HASAN,7 Nurul AKBAR,3 H.M. Sjaifoellah NOER,3 And Yoshitake HAYASHI1

1Departlnent of Anatomical Pathology, Faculty of Medicine, Indonesia University, Jakarta, Indonesia, 
2First Division, Department of Pathology, Kobe University School of Medicine, Kobe，Japan.
3Department of Internal Medicine, Faculty of Medicine, Indonesia University, Jakarta, Indonesia. 
4Departrment of Internal medicine, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia, 5Departrnent of Clinical Pathology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia, 
6Department of Anatomical Pathology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia,
7Department of Internal Medicine, Faculty of Medicine, Airlangga University, Surabaya, Indonesia 

Kobe J. Med. Sci. 46, 217-229, October 2000 

AB: Mutations of p53 as a tumor suppressor gene in hepatocellular carcinoma (HCC) have been reported to occur with varying frequency in different geographic regions, which might be different etiology for HCC. Overexpressions of p53 (well known for its implications in mutations of the p53 gene), PCNA and a-fetoprotein (AFP) have been reported to be associated with carcinogenesis and/or tumor progression and poor prognosis in various types of cancer. To estimate the geographical difference of the p53 gene, PCNA and AFP in HCC, we examined 14 Japanese HCC cases, 8 Indonesian HCC cases, and 27 Indonesian chronic active hepatitis (CAH) or liver cirrhosis cases, using immunohistochemical approaches. Overexpression of p53 was identified in 37.5% of Japanese HCC, in 62.5% of Indonesian HCC and none in CAH. The mean PCNA Labeling Index of Japanese HCC, Indonesian HCC and CAH was detected in 48.6%, 30.4%, and 43.5%, respectively. AFP was detected in 35.7% of Japanese and 25% of Indonesian HCC. The rate of p53 overexpression in Indonesian HCC was as high as in HCC of southern part of China, which might share the similar etiology in both regions.