Kobe Journal of Medical Sciences, 2000

EFFECTS OF ISCHEMIA AND REPERFUSION ON OXIDATIVE STRESS IN HEPATIC CIRRHOSIS INDUCED BY CARBON TETRACHLORIDE IN RATS.

Ernani Luis RHODEN1, Luiz, PEREIRA-LIMA1, Antonio Nochi KALIL1, 
Marcio Luis LUCAS2, Marcelo MAURI2, Eduardo MENTI2, Claudia Ramos RHODEN2, 
Jorge PEREIRA-LIMA3, Claudio Galeano ZETTLER4, and Adriane BELLO-KLElN5 

Department of General Surgeryl, Department of Pharrnaoology and Toxicology2 
Department of Gastroenterology3, Department of Pathology4; Porto Alegre School of 
Medical Sciences/Santa Casa University Hospital Department of Physiology, Federal University of Rio Grande do Sul5. porto Alegre，RS, Brazil 

Kobe J. Med. Sci. 46, 171-180, August 2000

AB: The present study was undertaken to determine the effect of ischemia and reperfusion on oxidative stress in hepatic cirrhosis induced by carbon tetrachloride (CCu) in rats by the evaluation of lipid peroxidation products (LPO). Cirrhosis of the liver was induced by CCu administration. This drug was dissolved in mineral oil and the control group received only mineral oil intraperitoneally. Forty-five minutes of ischemia followed by one hour of reperfusion were performed. LPO products were evaluated by the thiobarbituric acid reactive substances method (TBARS) and chemiluminescence initiated by tert-butyl hydroperoxide technique (CL). The liver was submitted to histologic evaluation to check whether cirrhosis was present. The results demonstrated that ischemia-reperfusion caused an increase of LPO products in cirrhotic rats when compared to the control group (p<0.05). Hepatic cirrhosis was present in all animals treated with CCL and no significant histologic alterations were observed in the control group. According to this study, we can conclude that the effect of ischemia and reperfusion in a rat model of hepatic cirrhosis caused a significant increase of the hepatic-levels of LPO products when compared to the noncirrhotic livers.