Kobe Journal of Medical Sciences, 1995
TI: Inhibitory effect of c-fos overexpression on B cell proliferative responses to membrane IGM cross-linking.
AU: Tomita-Y; Koizumi-T; Mizuno-K; Tokuhisa-T
AD: Department of Orthopedic Surgery, Kobe University School of Medicine.
SO: Kobe-J-Med-Sci. 1995 Oct; 41(5): 141-53
AB: Constitutive expression of the c-fos gene perturbs the de novo synthesis of RNA and DNA in B cells stimulated by surface immunoglobulin (sIg) cross-linking. In order to examine kinetics of the regulatory effect on the activation process of B cells, we used splenic B cells from transgenic mice carrying the c-fos gene under the control of the interferon alpha/beta (IFN)-inducible Mx gene promoter (Mx-c-fos). In the absence of IFN, Mx-c-fos B cells proliferated well by anti-IgM stimulation. However, both RNA and DNA synthesis in the Mx-c-fos B cells were markedly reduced by the addition of IFN in the culture within 12 h after anti-IgM stimulation. These results suggest that this regulatory effect of c-Fos displays at the mid G1 phase of the cell cycle.