Kobe Journal of Medical Sciences, 1993
TI: Analysis of enzyme-linked immunosorbent assay data for antigen quantitation using Lotus 1-2-3 software on a personal computer.
AU: Uehara-K; Konishi-E
AD: Department of Medical Zoology, Kobe University School of Medicine.
SO: Kobe-J-Med-Sci. 1993 Jun; 39(3): 107-22
AB: Absorbance data obtained in double-antibody sandwich enzyme-linked immunosorbent assay tests for quantitation of antigens are analyzed by a personal computer using the Lotus 1-2-3 software package. The sigmoid standard curve is processed by an iterative weighted regression analysis in the logit-log domain to obtain a linear dose-response relationship. A macro program is developed for quick calculation with easy operation, which is suited for routine laboratory tests in biomedical fields.