Kobe Journal of Medical Sciences, 1992
TI: Deregulated c-fos augments the expression of IL-2 gene in T cells stimulated with anti-CD3 antibody.
AD: Department of Immunology, ICMR, Kobe University School of Medicine.
SO: Kobe-J-Med-Sci. 1992 Dec; 38(6): 321-35
AB: The expression of IL-2 gene in T cells stimulated with immobilized anti-CD3 antibody is controlled by several transcription factors like as AP-1, IL-2 kappa B and NFAT. We studied an effect of deregulated c-fos, a component of AP-1, in the expression of IL-2 gene in T cells by using splenic T cells from transgenic mice carrying the exogenous c-fos gene under the control of the H2-Kb promoter (H2-c-fos). The IL-2 production of H2-c-fos T cells stimulated with anti-CD3 antibody was enhanced and prolonged. Levels of AP-1 and IL-2 kappa B transcription factors in the nuclear extract from the H2-c-fos T cells were also augmented. These results suggest that c-Fos is one of the major regulatory factors for the expression of IL-2 gene in T cells activated with anti-CD3 antibody.